Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Qualitative analysis of the staining on the cell array treated with the anti-Ep-CAM fluorescein-conjugated mAb. A: Reconstruction of the cell array using high-magnification images after superimposition of the fluorescein (Ep-CAM) and Hoecht (DNA) signal. B: Immunofluorescence staining with the anti-human Ep-CAM fluorescein conjugated mAb on the Hep3b and COLO205 cells. The different cell lines were positioned onto the array as follow: A2, BKGE; A3, COLO205; A4, HCT116; A5, SW620; B1, PC3; B2, DU145; B3, NRP154; B4, HIAEC; B5, HUVEC; C1, HMVEC; C2, NHDF; C3, CASMC; C4, NHEK; C5, A375; D1, SkBr3; D2, MCF7; D3, BT474; D4, A673; D5, HepG2; E1, Hep3b; E2, Jurkat; E3, Bjab; E4, SKMES1; and E5, U87MG.

Article Snippet: Normal cells included: human iliac artery endothelial cells (HIAECs), human umbilical vein endothelial cells (HUVECs), human microvascular endothelial cells from lung (HMVECs), normal human dermal fibroblasts (NHDFs), human coronary artery smooth muscle cells (CASMCs), and neonatal normal human epidermal keratinocytes (NHEKs) purchased from BioWhittaker/Clonetics (San Diego, CA) and the bovine kidney glomerular endothelial cell (BKGEs) purchased from VEC Technologies, Inc. (Rensselaer, NY, USA).

Techniques: Staining, Immunofluorescence

Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Binding of an anti-human Ep-CAM fluorescein-conjugated mAb to various cell lines analyzed by FACS. BKGEs (A2); human colorectal carcinoma cell lines COLO205 (A3), HCT116 (A4), and SW620 (A5); human prostate carcinoma cell lines PC3 (B1) and DU145 (B2); rat prostate tumorigenic cell line NRP154 (B3); HIAECs (B4); HUVECs (B5); lung HMVECs (C1); NHDFs (C2); human CASMCs (C3); neonatal NHEKs (C4); human malignant melanoma cell line A375 (C5); human breast carcinoma cell lines SkBr3 (D1), MCF7 (D2), and BT474 (D3); human rhabdomyosarcoma cell line A673 (D4); human liver carcinoma cell lines HepG2 (D5) and Hep3b (E1); T- and B-cell leukemia cell lines Jurkat (E2) and Bjab (E3), respectively; human lung carcinoma cell line SKMES1 (E4); and human neuroglioma cell line U87MG (E5) were incubated in the absence (thin line) or presence (thick line) of an anti-human Ep-CAM fluorescein-conjugated monoclonal antibody and analyzed by FACS.

Article Snippet: Normal cells included: human iliac artery endothelial cells (HIAECs), human umbilical vein endothelial cells (HUVECs), human microvascular endothelial cells from lung (HMVECs), normal human dermal fibroblasts (NHDFs), human coronary artery smooth muscle cells (CASMCs), and neonatal normal human epidermal keratinocytes (NHEKs) purchased from BioWhittaker/Clonetics (San Diego, CA) and the bovine kidney glomerular endothelial cell (BKGEs) purchased from VEC Technologies, Inc. (Rensselaer, NY, USA).

Techniques: Binding Assay, Incubation

Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Capture of the cell array fluorescent signal using different image acquisition systems. A: Hoechst fluorescent staining was analyzed for each spot using the same capture parameters on the fluorescence microscope and separate images were used to rebuild the complete cell array. B: Anti-human Ep-CAM fluorescein-conjugated mAb binding to the cell array analyzed using the same capture parameters on the fluorescence microscope. Separate images were used to rebuild the complete cell array. C and D: Anti-human Ep-CAM fluorescein-conjugated mAb binding to the cell array was analyzed using a Typhoon 8600 scanner or a white-light source CCD camera, respectively. Remarkably, the same array slide was used for the fluorescence microscopy and white-light source CCD camera analysis, whereas a different slide was used for the typhoon 8600 scanner analysis. The different cell lines were positioned onto the array as follows: A2, BKGE; A3, COLO205; A4, HCT116; A5, SW620; B1, PC3; B2, DU145; B3, NRP154; B4, HIAEC; B5, HUVEC; C1, HMVEC; C2, NHDF; C3, CASMC; C4, NHEK; C5, A375; D1, SkBr3; D2, MCF7; D3, BT474; D4, A673; D5, HepG2; E1, Hep3b; E2, Jurkat; E3, Bjab; E4, SKMES1; and E5, U87MG. Original magnifications, ×40.

Article Snippet: Normal cells included: human iliac artery endothelial cells (HIAECs), human umbilical vein endothelial cells (HUVECs), human microvascular endothelial cells from lung (HMVECs), normal human dermal fibroblasts (NHDFs), human coronary artery smooth muscle cells (CASMCs), and neonatal normal human epidermal keratinocytes (NHEKs) purchased from BioWhittaker/Clonetics (San Diego, CA) and the bovine kidney glomerular endothelial cell (BKGEs) purchased from VEC Technologies, Inc. (Rensselaer, NY, USA).

Techniques: Staining, Fluorescence, Microscopy, Binding Assay

Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Comparison of the FACS and the Cell Array Data Acquired Either with the Microscope Setup, a Typhoon 8600 Scanner, or a White Light Source CCD Camera

Article Snippet: Normal cells included: human iliac artery endothelial cells (HIAECs), human umbilical vein endothelial cells (HUVECs), human microvascular endothelial cells from lung (HMVECs), normal human dermal fibroblasts (NHDFs), human coronary artery smooth muscle cells (CASMCs), and neonatal normal human epidermal keratinocytes (NHEKs) purchased from BioWhittaker/Clonetics (San Diego, CA) and the bovine kidney glomerular endothelial cell (BKGEs) purchased from VEC Technologies, Inc. (Rensselaer, NY, USA).

Techniques: Comparison, Microscopy

Journal: Heliyon

Article Title: Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction

doi: 10.1016/j.heliyon.2017.e00369

Figure Lengend Snippet: (A) Representative images of in situ superoxide production. Blue or red fluorescence indicates nuclei and superoxide in the human coronary arterial smooth muscle cells, respectively. (B-D) Relative superoxide production in the human coronary arterial smooth muscle cells treated with the addition of L-glucose, D-glucose, D-glucose in combination with Tiron, gp91ds-tat, sgp91ds-tat, human serum albumin (BENESIS, 0.05 to 0.5 g/dL), methyl-β-cyclodextrin, gp60 antibody (20 μg/mL) or the combination for 60 min. (B) *: P < 0.05 vs. control. (C) *: P < 0.05 vs. D-glucose, and #: P < 0.05 vs. albumin (0.5 g/dL). (D) *: P < 0.05 vs. D-glucose.

Article Snippet: Human coronary arterial smooth muscle cells (Cat No. KS-4209, Kurabo Industries Ltd., Osaka, Japan) were grown in HuMedia-SG2 medium (Kurabo Industries Ltd., Osaka, Japan) at 37 °C in the humid air with 5% carbon dioxide.

Techniques: In Situ, Fluorescence, Control

Journal: Heliyon

Article Title: Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction

doi: 10.1016/j.heliyon.2017.e00369

Figure Lengend Snippet: (A) Relative protein expressions of NADPH oxidase subunits, p47phox, rac-1, NOX2, and Na + /K + ATPase in the membrane fraction from human coronary arterial smooth muscle cells 60 min after addition of L-, D-glucose (20 mmol/L) or D-glucose in combination with human serum albumin (BENESIS, 0.5 g/dL). *: P < 0.05 vs. L-glucose. Note that p47phox images split were taken from the same picture in the Supplementary Figure, where the full, non-adjusted images are available. (B) Immunofluorescent image analysis in the human coronary arterial smooth muscle cells 60 min after addition of L-, D-Glucose or D-Glucose in combination with human serum albumin (BENESIS, 0.5 g/dL) from five separate experiments. P47phox (green) was localized to the cytoplasmic region whereas it migrates to the cell periphery after incubation with D-glucose.

Article Snippet: Human coronary arterial smooth muscle cells (Cat No. KS-4209, Kurabo Industries Ltd., Osaka, Japan) were grown in HuMedia-SG2 medium (Kurabo Industries Ltd., Osaka, Japan) at 37 °C in the humid air with 5% carbon dioxide.

Techniques: Membrane, Incubation